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KMID : 0376219710080020223
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Chonnam Medical Journal
1971 Volume.8 No. 2 p.223 ~ p.237
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Studies on India Ink Absorption by Canal Epithelium of Rat Epididymis Correlating with Acid Phosphatase Activity
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Abstract
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In an attempt to investigate absorptive function of the epithelial cells of the epididymal canal in the rat, histological and histochemical studies, were carried out on the sections of epididymis after injection of india ink through the lumen of the vas deferens into the epididymal canal or by injecting into testis and following results were obtained.
1. From 6 hours after administration of the ink´ solution into the epididymis via the lumen of the vas deferens, the ink granules were appeared throughout the cytoplasm of only certain epithelial cells (1st type) in the terminal portion of epididymal canal, but were not found in majority of the epithelial cells (2nd type). Ratio of these phagocytic and nonphagocytic cells were not changed: in their occurrence according to the time from 6 up to 25 hours following administration of the solution. These indicate that the canal epithelium of the rat epididymis is composed of at least two functionally different cell types.
2. For the correlation of the cell types described above to the cell types reported previously with staining method for acid phosphatase, histochemical studies of the enzyme on the tissues from the epididymis, injected and uninjected the India ink solution were made. In the canal of these epididymis, two types of epithelial cells were identified and were constant in their ratio of the occurance in both cases of epididymis.
The first type cell in the uninfected epididymis, showed supranuclear activity of the enzyme which was slightly enhanced without change in its cytoplasmic distribution in the injected epididymis. Second cell type showed fine granular cytoplasmic activity of the enzyme often with nuclear staining in control side, however, the enzyme activity in the cells of the injected epididymis, was masked with black ink granules absorbed, indicating that these cells are identical with the cells of second type found in control epididymis.
Further more, ratio of first cell type to the seconds in the control epididymis were roughly equal to that of nonphagocytic to phagocytic cells of the India ink.
3. With PAS staining, two cell types also identified. One cell type contained supragranular PAS granules and the other type of cells contained PAS-granules throughout its cytoplasm. Ratio of the former to the later was estimated as being roughly equal to that of nonphagocytic and phagocytic cells of India ink solution.
4. To investigate the absorptive function of the ink by the canal epithelium in head and body portion of the rat epididymis, to which India ink had not reached with injection of the ink solution through vas deferens, India ink was injected into the rat testes and it was found that only one instance from 10 experiments showed few cells containing ink granules in a section of the head and body segment although most of cells in the interstices near the injected site of the testis contained abundant ink granules.
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